WO2013149761A1 - Élément capillaire pour récipient à réactifs et son utilisation - Google Patents

Élément capillaire pour récipient à réactifs et son utilisation Download PDF

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Publication number
WO2013149761A1
WO2013149761A1 PCT/EP2013/053406 EP2013053406W WO2013149761A1 WO 2013149761 A1 WO2013149761 A1 WO 2013149761A1 EP 2013053406 W EP2013053406 W EP 2013053406W WO 2013149761 A1 WO2013149761 A1 WO 2013149761A1
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WO
WIPO (PCT)
Prior art keywords
capillary
liquid
capillary structure
reagent vessel
force
Prior art date
Application number
PCT/EP2013/053406
Other languages
German (de)
English (en)
Inventor
Martina Daub
Guenter Roth
Nils Paust
Juergen Steigert
Original Assignee
Robert Bosch Gmbh
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Robert Bosch Gmbh filed Critical Robert Bosch Gmbh
Priority to EP13705186.8A priority Critical patent/EP2834005B1/fr
Publication of WO2013149761A1 publication Critical patent/WO2013149761A1/fr

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Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5021—Test tubes specially adapted for centrifugation purposes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/0406—Moving fluids with specific forces or mechanical means specific forces capillary forces
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/0409—Moving fluids with specific forces or mechanical means specific forces centrifugal forces
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/06—Valves, specific forms thereof
    • B01L2400/0688—Valves, specific forms thereof surface tension valves, capillary stop, capillary break

Definitions

  • the invention relates to a revolver component for a reagent vessel.
  • the invention likewise relates to a reagent vessel insertion part and a reagent vessel.
  • the invention relates to a method for centrifuging a material and to a method for pressure-treating a material.
  • the device constructed in the format of a standard centrifuge tube may comprise various turrets which are arranged axially one above the other.
  • the turrets may include channels, cavities, reaction chambers, and other structures for performing fluidic unit operations.
  • An integrated ballpoint pen mechanism allows the turrets to be rotated with respect to their positions relative to one another, as a result of which the structures of the revolvers can be switched to one another.
  • An update of the ballpoint pen mechanism is triggered after inserting the device in a centrifuge by means of a centrifugal force caused by the operation of the centrifuge. At the same time, liquids can be transferred along the force vector of the centrifugal force produced.
  • the invention provides a revolver component for a reagent container with the features of claim 1, a reagent container insert part having the features of claim 10, a reagent container for a centrifuge and / or for a pressure varying device with the features of claim 1 1, a method for centrifuging a A material having the features of claim 12 and a method of pressure treating a material having the features of claim 14.
  • the capillary force brought about by the at least one capillary structure can be used to move the at least one liquid within the at least one vessel structure out of the at least one vessel structure and / or into the at least one vessel structure
  • Transfer vessel structure of the revolver component As will be explained in more detail below, by means of the capillary force also a liquid transport against a centrifugal force caused by the operation of a centrifuge and / or against a pressure force caused by an operation of a pressure-varying device can be realized.
  • the capillary force of the at least one capillary structure can also be advantageously used to temporarily store the at least one liquid.
  • the at least one capillary structure in and / or on the revolver component is thus an advantageous
  • Control component for controlling a liquid transport of the at least one liquid and / or storing the at least one liquid.
  • the at least one capillary structure is also used as a passive valve structure and / or passive mixing component for mixing
  • Fluids can be used.
  • the at least one capillary structure and / or in the revolver component can thus be used for a variety of uses.
  • the at least one capillary structure has an average diameter in a range between 0.1 ⁇ m to 1 mm.
  • the at least one mean diameter may in particular be in a range between 1 ⁇ to 100 ⁇ . This ensures a sufficiently high capillary force of the at least one capillary structure, by means of which the at least one liquid (optionally) can be sucked into the internal volume of the at least one capillary structure.
  • the at least one capillary structure may be formed of glass, silica (silica gel), a polymer, a fabric material and / or a gel.
  • the at least one capillary structure is thus relatively simple and inexpensive executable.
  • the at least one capillary structure is coated on its at least one inner wall with proteins, antigens, antibodies, enzymes, DNA partial strands, RNA partial strands and / or epoxy resin.
  • the at least one capillary structure is thus versatile.
  • the capillary force which can be exerted by means of the at least one capillary structure is greater than a weight force of the at least one liquid which can be filled or filled into the at least one vessel structure.
  • the at least one capillary structure can thus be used to transfer the at least one liquid counter to the weight and / or despite the weight
  • the revolver component has a revolver outer wall, which is designed such that the revolver component can be inserted in a reagent vessel for a centrifuge and / or for a pressure-varying device.
  • a revolver outer wall which is designed such that the revolver component can be inserted in a reagent vessel for a centrifuge and / or for a pressure-varying device.
  • Reagent vessel insert part can be used, which is designed so that the
  • Reagent vessel insert part can be used in a reagent vessel for a centrifuge and / or for a Druckvariiervoriques.
  • the revolver component can thus be advantageously used during centrifuging, applying an overpressure and / or applying a negative pressure.
  • the at least one capillary structure is therefore both a passive valve structure for switching Liquids, as well as a passive mixing component for mixing liquids.
  • the at least one vessel structure comprises in each case at least one first chamber with a filling and / or pressure compensation opening and a second chamber which is air-tight and / or liquid-tight except for a liquid exchange opening to the first chamber, wherein the at least one Capillary structure is formed in a formed as a spongy mass capillary system, which is arranged in the second chamber.
  • Reagent vessel insertion part can be realized, which has a Einskyeilgephaseuse which is designed so that the Reagenzgefäß-insert part is insertable in a reagent vessel for a centrifuge and / or for a Druckvariiervoriques, and which has at least one arranged in the Einskyeilgephaseuse turret component according to the inventive technology.
  • these advantages can be ensured by a reagent vessel for a centrifuge and / or for a pressure varying device with at least one turret component arranged in the reagent vessel according to the technology according to the invention.
  • the method has the additional steps: At least one intermediate reduction of the current time
  • Rotary speed to a second desired rotational speed which causes a second centrifugal force less than the capillary force of the at least one capillary structure, whereby the material to be centrifuged and / or the other liquid are at least partially sucked into the at least one capillary structure, and increasing the current rotational speed to one third desired rotational speed which causes a third centrifugal force greater than the capillary force of the at least one capillary structure.
  • the method may have the additional steps: At least one adjustment of the lower or Overpressure in the direction of the atmospheric pressure to a second desired pressure, which causes a second pressure force less than the capillary force of the at least one capillary structure, whereby the material and / or the other liquid are at least partially sucked into the at least one capillary structure, and reinforcing the or excess pressure away from the atmospheric pressure to a third target pressure which causes a third pressure force greater than the capillary force of the at least one capillary structure
  • FIG. 1 a and 1 b are schematic representations of a first embodiment of the
  • 3a and 3b are schematic representations of a third embodiment of the
  • 4a to 4c are schematic representations of a fourth embodiment of the
  • 5a to 5c are schematic representations of a fifth embodiment of the
  • Fig. 6 is a schematic representation of an embodiment of the
  • FIG. 8 is a flowchart for explaining an embodiment of the present invention
  • FIGS. 1 a and 1 b show schematic representations of a first embodiment of the revolver component.
  • the turret component 10 shown schematically in FIGS. 1a and 1b can be used in a reagent vessel.
  • the revolver component 10 may have a turret outer wall 12, which is designed so that the revolver component 10 can be inserted in a reagent vessel for a centrifuge and / or for a pressure-varying device.
  • a turret outer wall 12 As an alternative or in addition to the turret component 10 due to its turret outer wall 12 in a Einticianeilgephase a
  • Reagent vessel insert part can be used, which is designed so that the
  • Reagent vessel insert part can be used in a reagent vessel for a centrifuge and / or for a Druckvariiervoriques.
  • the applicability of the turret member 10 / of the reagent vial inserter to the subject reagent vial for a centrifuge and / or a pressure varying device may be interpreted as meaning that the turret outer wall 12 / an outer wall of the insert member housing corresponds to an inner wall of the reagent vial.
  • the turret outer wall 12 / the outer wall of the Einassieilgephaseuses contacted the inner wall of the reagent vessel such that even during operation of the centrifuge and / or the Druckvariiervorraum a reliable grip of the turret member 10 / the Reagenzgefäß-insert in the relevant
  • Reagent vessel is guaranteed.
  • Test tube / test tube understood.
  • Other embodiments include centrifuge tubes, 1.5 ml Eppendorf tubes, 2 ml Eppendorf tubes, 5 ml Eppendorf tubes and microtiter plates, such as e.g. 20 ⁇ _ microtiter plates (per well).
  • the reagent vessel can be a test carrier or a disposable cartridge, which are designed as a lab-on-a-chip system on a plastic-plastic-sized plastic substrate.
  • the formability of the reagent vessel is not limited to the examples listed here.
  • the dimensions of the reagent vessel are only due to a desired applicability of
  • the reagent vessel in the centrifuge and / or specified in the Druckvariiervorraum.
  • the feasibility of the technologies according to the invention described below however, does not prescribe any external shape of the reagent vessel.
  • the reagent vessel can be designed to receive samples in an amount which can be selected from a range of a few ⁇ _ to 1 L, optionally. It should be noted that under the below-mentioned centrifuge and
  • the revolver component 10 can be understood in particular a turret for a reagent vessel.
  • the revolver component 10 may e.g. be designed so that it is rotatable about a rotation axis 1 1 by means of a suitable mechanism which can be arranged on the turret component 10 or separated from the turret component 10.
  • the axis of rotation 1 1 can in particular run centrally through the turret component 10 and / or be aligned perpendicular to the at least one vessel bottom.
  • the revolver component 10 / the reagent vessel insertion part can also be designed for interaction with a ballpoint pen mechanism or comprise a ballpoint pen mechanism.
  • the turret member 10 / reagent vial insert may hold a volume less than 5 milliliters.
  • the revolver component 10 can thus be designed in particular such that it can be integrated in a stack of further revolvers and / or reaction chambers.
  • turrets, reaction chambers and / or cavities axially stacked one above the other
  • At least one vessel structure 14, into which at least one liquid 16 can be filled or filled, is formed on the revolver component 10. The at least one
  • Liquid 16 may be, for example, a material / sample material to be examined and / or at least one chemical. It should be noted that the turret component 10 described below is not based on the use of certain
  • Liquids is limited. In addition, at the turret component 10 more
  • Vascular structures 14 may be formed, which extend from the axis of rotation 1 1 radially to the turret outer wall 12.
  • the practicability of the revolver component 10 is not limited to a specific shape of the at least one vessel structure 14 and / or a certain number of vessel structures 14 of the turret component 10.
  • the revolver component 10 has at least one capillary structure 18 arranged on and / or in the at least one vessel structure 14, by means of which a capillary structure 18 is provided
  • Capillary force on the at least one liquid 16 is exercisable.
  • the at least one liquid 16 is at least partially sucked into an internal volume 20 of the at least one capillary structure 18.
  • the at least one capillary structure 18 allows at least temporary storage of the at least one liquid 16 sucked therein.
  • the at least one capillary structure 18 can thus result in liquid transport of the at least one liquid 16 within the at least one vessel structure 14, out of the at least one vessel structure 14, and / or in the at least one
  • Vascular structure 14 are used in it. Furthermore, the at least one
  • Capillary structure 18 an advantageous memory component for
  • the at least one capillary structure 18 has a mean diameter, which is in a range between 0.1 ⁇ to 1 mm.
  • the average diameter of the at least one capillary structure 18 can be in a range between 1 ⁇ m to 500 ⁇ m, preferably between 1 ⁇ m and 100 ⁇ m. This can also be described in such a way that the at least one capillary structure 18 has a pore size in a range between 0.1 ⁇ m to 1 mm.
  • the at least one capillary structure 18 may be formed, for example, of glass, silica, a polymer such as polyester, polypropylene, polytetrafluoroethylene, nylon, and / or polyvinylidene fluoride, a fabric cloth, and / or a gel.
  • the at least one capillary structure 18 may be formed in particular as a glass filter.
  • the designability of the at least one capillary structure 18 is not limited to the materials listed here.
  • Capillary structure 18 may also be formed from a turret material of the turret component 10. Although it is advantageous due to the simple equipment of the turret component 10 with the at least one capillary structure 18 to use the turret material as a capillary material, the manufacturability of at least one
  • Capillary structure but not on the one-piece forming the at least one Capillary structure 18 is limited to the turret component 10 by means of a casting process or an injection molding process.
  • the revolver component 10 can also first be formed without the at least one capillary structure 18 and then be equipped with the at least one capillary structure 18.
  • the at least one capillary structure 18 can also have a continuous, discontinuous and / or defined geometry.
  • the at least one capillary structure 18 may have, for example, a round channel cross section, a quadrangular channel cross section and / or a polygonal channel cross section.
  • the at least one capillary structure 18 can be used individually or as a bundle. Instead of a single capillary structure 18 or a defined number of
  • Capillary structures 18, the turret component also a capillary system of flow, filter, columnar and sponge-like capillary 18 have.
  • the turret member 10 may be made in one piece by means of a casting method or an injection molding method despite its advantageous usability.
  • Revolver component 10 is thus inexpensive to produce.
  • the internal volume of the Revolver component 10 is thus inexpensive to produce.
  • Revolver component 10 / of the reagent vessel insert may be at least partially made of a polymer, e.g. from COP, COC, PC, PA, PU, PP, PET and / or PMMA. Other materials are used to form the interior volume of the turret component 10 /
  • the at least one capillary structure 18 is coated on its at least one inner wall 22 with proteins, antigens, antibodies, enzymes, DNA partial strands, RNA partial strands and / or epoxy resin. This can also be done as an immobilization of the at least one inner wall 22 of the at least one
  • Capillary structure 18 rewrite with biological probes.
  • the at least one capillary structure 18 for transferring / transporting, buffering, storing, retaining and / or mixing the at least one liquid 16 biochemical / molecular biological reactions, in particular specific protein and / or DNA bonds, enzymatic reactions and / or or DNA hybridizations are performed.
  • unspecific bindings can be prevented during the processes described here. This extends the applicability of the at least one capillary structure 18.
  • Capillary structure 18 also be coated / modified so that their
  • wetting properties and / or their contact angle cause a particularly high capillary force on the at least one liquid 16.
  • the at least one inner wall 22 of the at least one capillary structure 18 due to their
  • the at least one inner wall 22 of the at least one capillary structure 18 can have a comparatively high roughness for this purpose.
  • the capillary force exerted by means of the at least one capillary structure 18 (on the at least one liquid 16) is greater than a weight of the at least one liquid 16 which can be filled or filled in the at least one vessel structure 14.
  • the at least one at least partially sucked into the at least one capillary structure 18 Liquid 16 can be effected by means of a centrifugal force which can be effected during operation of the centrifuge, in whose rotor device the reagent vessel is arranged with the revolver component 10 inserted therein, and / or by means of a centrifugal force during operation of the pressure varying device in which the reagent vessel with the revolver component 10 inserted therein is arranged; be effected compressive force from the at least one capillary 18 out transferable.
  • the capillary force which by means of the at least one capillary structure 18 on the at least one
  • Liquid 16 is exercisable, a centrifugal acceleration of at most 1000 g, at most 500 g, in particular at most 200 g, correspond.
  • a centrifugal acceleration of at most 1000 g, at most 500 g, in particular at most 200 g correspond.
  • the at least one liquid 16 sucked into the at least one capillary structure 18 can be pressed out again in a simple manner.
  • the at least one liquid 16 temporarily stored in the at least one capillary structure 18 can easily be transferred out of this again.
  • the at least one capillary structure 18 is formed as a curved capillary.
  • the at least one capillary structure 18 has an intake opening 24 and an outlet opening 26, the outlet opening 26 being movable with respect to a direction of action 28 of a centrifugal force which can be effected by means of a centrifuge and / or a compressive force which can be effected by means of a pressure-varying device
  • the at least one capillary structure 18 makes it possible to suck the at least one liquid 16 against a direction of action 28 of the centrifugal force which can be effected by means of a centrifuge and / or the pressure force which can be effected by means of a pressure-varying device.
  • the at least one liquid 16 can be reliably sucked into the at least one capillary structure 18. If the centrifugal force and / or pressure force exerted on the liquid 16 filled in the inner volume 20, on the other hand, exceeds the capillary force of the at least one capillary structure 18, the advantageous shape of the at least one capillary structure 18 causes a first liquid flow 30 of a first one
  • the first quantity of liquid corresponds to a first partial volume of the at least one capillary structure 18, which extends from the respective intake opening 24 to a (virtual) parting plane 34.
  • the respective separating plane 34 intersects the associated capillary structure 18 at a point 36 which is directed furthest towards the direction of action 28 and which can also be rewritten as a high point of the respective capillary structure 18.
  • the second liquid quantity is defined by a second partial volume of the at least one capillary structure 18, which extends from the respective outlet opening 26 to the parting plane 34.
  • the first subvolume and the second subvolume may together provide the interior volume 20 of the at least one capillary structure 18.
  • the embodiment described here of the at least one capillary structure 18, provided that the second amount of liquid flowing out of the outlet opening 26 again into a common vessel with the from the
  • Intake port 24 exiting first amount of liquid is introduced are used as a mixer. It is pointed out in particular that the at least one advantageous capillary structure 18 permits a passive mixing of the at least one liquid 16 even without a mechanical / adjustable element or without a movable part.
  • the at least one capillary structure 18 can also be used for measuring a defined amount of liquid and for transporting the defined amount of liquid into a desired target volume. Since the volume and / or the shape of the at least one capillary structure, the first amount of liquid and the second
  • Liquid quantity can be determined precisely, thus by means of the embodiment shown with reference to FIGS. 1 a and 1 b liquid volumes (exactly) and, if desired, be passed into separate chambers.
  • the at least one capillary structure 18 designed as a riser capillary can also have a plurality of outlets in order to meter off a plurality of partial volumes in parallel. (By means of such a riser capillary also a mixing efficiency can be increased).
  • At least one channel, at least one cavity and / or at least one reaction chamber may be formed in the revolver component 10 / a reagent vessel insertion part equipped therewith.
  • Process steps and structures such as, for example, sedimentation structures, channel structures or siphon structures for forwarding and switching at least one in the revolver component 10, can be integrated in the inner volume of the revolver component 10 / of the reagent vessel insertion part
  • Reagent vessel insert part contained liquid.
  • at least one further subunit of the inner volume of the revolver component 10 / of the reagent vessel insertion part can be filled with at least one liquid as a "storage container", which can be filled, processed and / or examined subsequently
  • Material / sample material at least one chemical reaction and / or one
  • Reservoir may be used, for example, with chemicals (e.g., buffers), enzymes, lyphilisates, beads, dyes, antibodies, antigens, receptors, proteins, DNA strands, and / or
  • the turret member 10 / reagent vial insert may also be equipped with additional components such as valves and / or pumps.
  • the technology according to the invention can also interact with a multiplicity of conventional actuation, detection and / or control units.
  • FIGS. 2a to 2c show schematic representations of a second embodiment of the revolver component.
  • the turret component 10 shown schematically (at least partially) in FIGS. 2 a to 2 c has a capillary system 40 instead of a limited number of capillary structures in which a plurality of capillary structures is formed.
  • the capillary system 40 may be formed, for example, as a filter.
  • FIG. 2 a shows the revolver component 10 immediately after a filling of the at least one liquid 16 by a
  • the at least one liquid 16 contacts the capillary system 40, which delimits a chamber 44 formed in the respective vessel structure 14 from the filling opening 42.
  • the at least one liquid 16 is then sucked into the capillary system 40, wherein the capillary force prevents leakage of the at least one liquid 16 into the chamber 44 (despite a weight force of the at least one liquid 16) (see Fig. 2b).
  • the at least one liquid 16 is thus incubable, the capillary system 40 being usable as incubation chamber / reaction chamber.
  • the capillary force which can be exerted by means of the capillary system 40 can be greater than the weight force of the at least one aspirated liquid 16.
  • the achievable capillary force may be greater than a centrifugal force and / or compressive force below a predetermined threshold.
  • 40 incubation times can be maintained by means of the capillary system, which can optionally have a duration of a few milliseconds to minutes or hours.
  • the capillary system 40 thus enables a sequential (optional) switching of a liquid flow, which is directed from the filling opening 42 into the chamber 44.
  • the at least one liquid for a definable / defined holding time which may be in the range of a few milliseconds to hours, are temporarily stored in the capillary system 40.
  • Capillary system can be used without a mechanical opening or closing mechanism. Therefore, the capillary system 40 is inexpensive due to its low cost
  • the capillary system 40 can also be used as a pressure and / or flow restrictor with increased fluidic resistance.
  • FIGS. 3a and 3b show schematic representations of a third embodiment of the revolver component.
  • Capillary 40 through the at least one filling opening 42 filled with at least one second liquid 46. Due to the increased hydrostatic pressure which is exerted on the at least one first liquid 16 by means of the at least one second liquid 46, the at least one first liquid 16 can be displaced from the capillary system 40, which is filled with the at least one second liquid 46. Optionally, for displacing the at least one first liquid 16 from the capillary system 40, a (comparatively small) centrifugal force / pressure force can be used to assist.
  • the at least one second liquid 46 is selected such that the capillary force exerted thereon by means of the capillary system 40 is greater than the capillary force
  • FIGS. 4a to 4c show schematic representations of a fourth embodiment of the revolver component.
  • the turret component 10 shown schematically (at least partially) in FIGS. 4 a to 4 c has at least one vessel structure 14, which in each case comprises at least one first chamber 50 with a filling and / or pressure compensation opening 52 and a second chamber 54, the second chamber 54 except for a liquid exchange port 56, via which it is hydraulically connected to the first chamber 50, air and / or
  • FIG. 4 a shows the revolver component 10 immediately after the at least one liquid 16 has been filled, for example through the flow and / or pressure compensation opening 52.
  • the at least one liquid 16 is absorbed by the capillary system 40 relatively quickly after it has been introduced. This can lead to (almost) complete filling of the second chamber 54. For example, in this way, the first chamber 50 (almost) completely emptied.
  • the turret member 10 is insertable (in a reaction vessel) so that the liquid exchange port 56 during operation of the centrifuge / Druckvariiervoriques one in the direction of the Aktuationskraft Fa aligned
  • Part of the first chamber 50 connects with a direction of the Aktuationskraft Fa aligned portion of the second chamber 54.
  • the advantage described below is also ensured if the first chamber 50 is aligned with respect to the second chamber 55 in the direction of the actuation force Fa.
  • orientation of a partial region in the direction of the actuation force Fa can be understood to mean that the partial region is aligned relative to a remaining region of the associated chamber 50 or 54 to the tip of a vector representing the actuation force Fa
  • the vector representing the actuation force Fa extends from the second chamber 54 to the first chamber 50
  • Part of the first chamber 50 connects with a direction of the Aktuationskraft Fa aligned portion of the second chamber 54, the filling of the capillary system 40, as long as a liquid column in the first chamber 50 is higher than a liquid column in the second chamber 54, by means of the Aktuationskraft Fa actively supported.
  • an applied actuation force Fa less than a capillary force Fk causes no disturbance of the intermediate storage in the capillary system 40
  • an actuation force Fa also causes smaller than the capillary force Fk of the capillary system 40, which can be exerted on the at least one liquid 16 sucked into the capillary system 40 by means of an operation of a centrifuge and / or a pressure-varying device is not emptying the
  • the capillary system 40 Only from an actuation force Fa greater than the capillary force Fk, the at least one liquid 16 sucked in previously by the capillary system 40 is ejected (see FIG. 4c). In this way, by means of the interaction of the forces Fa and Fk fluidic unit operations can be realized. As long as the actuation force Fa is smaller than the capillary force Fk of the capillary system 40, the capillary system 40 can be used as a valve controlled in a closed state. By adjusting an actuation force Fa greater than the capillary force Fk, the valve can be controlled in its open state. Reducing the actuation force Fa again below the capillary force Fk can lead to (reversible) control / switching of the valve into its closed state. It should be noted that the threshold from which the valve is controllable from a closed state to an open state is optional
  • a threshold value of 20 g can be set by means of a spongy capillary system 40. By applying a coating / modification on the inner walls of the capillary system 40, this threshold can be increased to 5000 g.
  • 5a to 5c show schematic representations of a fifth embodiment of the revolver component.
  • the turret component 10 (at least) partially schematically illustrated in FIGS. 5a to 5c has, as a further development, an additional third chamber 58, which is hydraulically connected to the first chamber 50 except for a liquid exchange opening 60 , air and / or liquid-tight is completed.
  • a further capillary system 40 which may be formed according to the embodiments described above, also arranged.
  • the turret component 10 described here has an obstacle structure 62, which is arranged between the filling and / or pressure equalization opening 52 and the two liquid exchange openings 56 and 60.
  • the obstacle structure 62 may be formed, for example, as a sieve.
  • the at least one liquid 16 After filling at least one liquid 16 into the first chamber 50 through the filling and pressure equalizing opening 52 (see FIG. 5 a), the at least one liquid 16 is sucked into the two capillary systems 40. If no actuation force Fa is exerted on the at least one fluid 16, which is greater than the capillary force Fk of the capillary systems 40, the aspiration process 64 shown schematically in FIG. 5 b is carried out continuously and the at least one aspirated fluid 16 remains stored in the two capillary systems 40 , However, if the actuation force Fa exerted on the at least one aspirated liquid 16 exceeds the capillary force Fk of the two capillary systems, then the at least one liquid 16 will be out of the
  • Liquids are used. For example, a sample may be aspirated in the second chamber 54 while the remainder of the process liquid is stored as a waste liquid in the third chamber 58. Because process liquids usually
  • Capillary system 40 of chamber 58 therefore functions similarly to a suction sponge or superabsorber.
  • the capillary system 40 can be designed to be elastic.
  • the capillary system 40 may be compressible.
  • the at least one aspirated liquid 16 can not only be ejected out of it by means of deforming / compressing the at least one capillary system 40, but can also be actively squeezed out. This effect can be amplified by acting on one of the direction of the Aktuationskraft Fa opposite end / radially inner end of the
  • Capillary system 40 at least one additional mass is attached.
  • the pressing out of the at least one can be any one of the at least one.
  • the at least one liquid 16 can be transferred out of the at least one capillary system 40 even with an actuation force Fa smaller than the capillary force Fk.
  • Capillary system 40 and the resulting liquid stream 66 can be mixed at least two liquids 16.
  • the mixing efficiency can be further increased. It should be noted that the at least one obstacle structure 62 is both stationary in the
  • Revolver component 14 can be designed as well as movable.
  • Fig. 6 shows a schematic representation of an embodiment of the Reagenzgefäß- inserting part.
  • the reagent vessel insertion part 70 shown schematically in FIG. 6 has a
  • Insert part housing 72 which is formed so that the reagent vessel insertion part 70 is insertable in a reagent vessel for a centrifuge and / or for a Druckvariiervoriques.
  • the applicability of the reagent vessel insertion part 70 into the relevant reagent vessel for a centrifuge and / or a pressure-varying device can be interpreted such that an outer wall 74 of the insertion part housing 72 corresponds to an inner wall of the reagent vessel.
  • the reagent vessel insertion part 70 includes at least one in the
  • Insert part housing 72 arranged turret component 10a, 10b and 10c.
  • the at least one revolver component 10a, 10b and 10c may be designed to be around the
  • Rotary axis 1 1 is rotatable.
  • the at least one turret component 10a, 10b and 10c can also be adjustable along the axis of rotation 11 (lateral). In this way, a distance between adjacent turret components 10a, 10b and 10c can be varied.
  • the lateral adjustability of the at least one revolver component 10a, 10b and 10c can be effected, for example, by means of a ball-point pen mechanism 76, which is shown only schematically in FIG. (Components of the ballpoint pen mechanism may for example be formed as part of the first turret component 10a and / or the second turret component 10b.)
  • a ball-point pen mechanism 76 can also be used a deformable polymer / elastomer, a
  • restoring force which is a return of at least one
  • Starting position / initial position causes.
  • a compressible material such as a polymer
  • a stretchable material which generates a tensile force which, as the restoring force, causes the at least one turret component 10a, 10b and 10c to be returned to a starting position / starting position.
  • the embodiments described in the upper paragraphs can also be combined with each other differently.
  • Reagent vessel insertion part also for a reagent vessel for a centrifuge and / or a Druckvariiervoriques, which is formed according to the explained reagent vessel insertion parts.
  • the advantageous reagent vessel has an outer wall which is designed so that the reagent vessel can be used in a centrifuge and / or in a pressure-varying device.
  • the reagent vessel is designed so that a reliable hold of the reagent vessel in the operated centrifuge and / or in the operated
  • a reagent vessel for a centrifuge and / or a pressure variegating device can thus be understood to mean a reagent vessel which, due to its (outer) shape, lends itself well to operation of the centrifuge with a comparatively high rotational speed and / or for application of a pressure deviating greatly from the atmospheric pressure - And / or negative pressure by means of
  • the advantageous reagent vessel can be vascular structures, such as channels, reaction chambers, storage chambers and / or active
  • reaction vessel may comprise actuation, detection and control units.
  • Reagent vessel can thus chemical reactions and / or
  • FIG. 7 is a flow chart for explaining an embodiment of the method for centrifuging a material.
  • Reagent filled with a turret component inserted therein Reagent filled with a turret component inserted therein.
  • Revolver component which also after filling the material in the
  • Reagent vessel can be introduced, is equipped with the advantageous technology.
  • the turret components described above may be used to carry out the method. The feasibility of here
  • the material to be centrifuged and / or the other liquid are at least partially transferred out of the at least one capillary structure.
  • the method also includes the method steps S2 and S3, which are each carried out at least once.
  • the current rotational speed is temporarily reduced to a second desired rotational speed, which causes a second centrifugal force less than the capillary force of the at least one capillary structure, whereby the material to be centrifuged and / or the other fluid at least partially into the at least one capillary structure be sucked in.
  • Method step S3 increases the current rotational speed to a third desired rotational speed, which causes a third centrifugal force greater than the capillary force of the at least one capillary structure.
  • a repeated execution of the method steps S2 and S3 can be used for mixing a plurality of liquids and / or for pumping liquid
  • Fig. 8 is a flow chart for explaining an embodiment of the method for pressure-treating a material.
  • the material to be treated by means of an underpressure or an overpressure, for example a sample material, is placed in a reagent vessel with one therein
  • step S10 used turret component filled (step S10).
  • the turret components described above may be used to carry out the method.
  • the feasibility of the method described here is not limited to the onset of these turret components.
  • a negative pressure or superatmospheric pressure corresponding to a first desired pressure is applied, which effects a first pressure force on the material and / or another liquid filled into the reagent vessel, which is greater than the capillary force of the at least one capillary structure.
  • the method also has the method steps S12 and S13, which can be repeated as often as desired.
  • the underpressure or overpressure is adjusted in the direction of
  • Atmospheric pressure to a second desired pressure, which causes a second pressure force smaller than the capillary force of the at least one capillary structure, which is why the material and / or the other liquid are at least partially sucked into the at least one capillary structure.
  • Process step S13 the negative or positive pressure away from the atmospheric pressure to a third target pressure, which is a third pressure force greater than the
  • Capillary force causes the at least one capillary, be strengthened.
  • a complete mechanical and / or fluidic functionality can be formed, which can be used for the automation of complex chemical processes and / or biochemical / molecular biological processes.
  • Automation can also be used to detect substances.
  • valve operations and / or mixing operations may also be performed by the methods. It should also be understood that the methods may also be used to include at least one liquid without a mechanical element and / or a movable one Part against an actuation force Fa, such as a centrifugal force and / or a compressive force to transport.

Abstract

La présente invention concerne un élément rotatif (10) destiné à un récipient à réactifs, au moins une structure de récipient (14) étant formée contre l'élément rotatif (10), ladite structure étant ou pouvant être remplie d'au moins un liquide (16), et l'élément rotatif (10) présentant au moins une structure capillaire (18) qui est disposée contre et/ou dans la au moins une structure de récipient (14) et au moyen de laquelle une force de capillarité peut être exercée sur le ou les liquides (16), ce qui permet au(x) liquide(s) (16) d'être aspiré(s) au moins en partie dans le volume intérieur (20) de la au moins une structure capillaire (18). L'invention porte également sur un insert pour récipient à réactifs et un récipient à réactifs. L'invention concerne aussi un procédé pour centrifuger une matière et un procédé pour traiter une matière sous pression.
PCT/EP2013/053406 2012-04-04 2013-02-21 Élément capillaire pour récipient à réactifs et son utilisation WO2013149761A1 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
EP13705186.8A EP2834005B1 (fr) 2012-04-04 2013-02-21 Dispositif capillaire pour récipient pour réactif et l'utilisation

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE102012205511.8 2012-04-04
DE102012205511A DE102012205511A1 (de) 2012-04-04 2012-04-04 Revolverbauteil für ein Reagenzgefäß

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WO2013149761A1 true WO2013149761A1 (fr) 2013-10-10

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DE (1) DE102012205511A1 (fr)
WO (1) WO2013149761A1 (fr)

Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1989000458A1 (fr) * 1987-07-17 1989-01-26 Martin Marietta Energy Systems, Inc. Rotor permettant le traitement de liquides au moyen de tubes capillaires mobiles
WO1990015321A2 (fr) * 1989-05-30 1990-12-13 Martin Marietta Energy Systems, Inc. Rotor et methode de traitement automatique des liquides a des fins de laboratoire et d'analyse biologique
GB2268911A (en) * 1992-07-24 1994-01-26 Canon Kk Capillary control of ink flow in ink containers for jet printers
US5789259A (en) * 1996-09-27 1998-08-04 Robert A. Levine Method and apparatus for mixing samples in a capillary tube
WO2008064783A1 (fr) * 2006-11-27 2008-06-05 Genedia S.R.L. Réacteur pour effectuer des procédés biochimiques
WO2010101950A1 (fr) * 2009-03-02 2010-09-10 Catholic Healthcare West Dispositifs diagnostiques et procédés d'utilisation
DE102010003223A1 (de) 2010-03-24 2011-09-29 Albert-Ludwigs-Universität Freiburg Vorrichtung zum Einsetzen in einen Rotor einer Zentrifuge, Zentrifuge und Verfahren zum fluidischen Koppeln von Kavitäten

Patent Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1989000458A1 (fr) * 1987-07-17 1989-01-26 Martin Marietta Energy Systems, Inc. Rotor permettant le traitement de liquides au moyen de tubes capillaires mobiles
WO1990015321A2 (fr) * 1989-05-30 1990-12-13 Martin Marietta Energy Systems, Inc. Rotor et methode de traitement automatique des liquides a des fins de laboratoire et d'analyse biologique
GB2268911A (en) * 1992-07-24 1994-01-26 Canon Kk Capillary control of ink flow in ink containers for jet printers
US5789259A (en) * 1996-09-27 1998-08-04 Robert A. Levine Method and apparatus for mixing samples in a capillary tube
WO2008064783A1 (fr) * 2006-11-27 2008-06-05 Genedia S.R.L. Réacteur pour effectuer des procédés biochimiques
WO2010101950A1 (fr) * 2009-03-02 2010-09-10 Catholic Healthcare West Dispositifs diagnostiques et procédés d'utilisation
DE102010003223A1 (de) 2010-03-24 2011-09-29 Albert-Ludwigs-Universität Freiburg Vorrichtung zum Einsetzen in einen Rotor einer Zentrifuge, Zentrifuge und Verfahren zum fluidischen Koppeln von Kavitäten

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EP2834005B1 (fr) 2019-05-08
DE102012205511A1 (de) 2013-10-10

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