WO2014056896A3 - One-step biomolecular immobilisation procedure and products thereof - Google Patents

One-step biomolecular immobilisation procedure and products thereof Download PDF

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Publication number
WO2014056896A3
WO2014056896A3 PCT/EP2013/070919 EP2013070919W WO2014056896A3 WO 2014056896 A3 WO2014056896 A3 WO 2014056896A3 EP 2013070919 W EP2013070919 W EP 2013070919W WO 2014056896 A3 WO2014056896 A3 WO 2014056896A3
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WO
WIPO (PCT)
Prior art keywords
immobilisation
products
biomolecular
biomolecule
immunoassays
Prior art date
Application number
PCT/EP2013/070919
Other languages
French (fr)
Other versions
WO2014056896A2 (en
Inventor
Sandeep Kumar VASHIST
Günter Roth
Jürgen Burger
Gregor CZILWIK
Original Assignee
Albert-Ludwigs-Universität Freiburg
Hsg-Imit Institut Für Mikro- Und Informationstechnologie
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Albert-Ludwigs-Universität Freiburg, Hsg-Imit Institut Für Mikro- Und Informationstechnologie filed Critical Albert-Ludwigs-Universität Freiburg
Priority to EP13773780.5A priority Critical patent/EP2903740A2/en
Publication of WO2014056896A2 publication Critical patent/WO2014056896A2/en
Publication of WO2014056896A3 publication Critical patent/WO2014056896A3/en

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Classifications

    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K14/00Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/415Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54353Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals with ligand attached to the carrier via a chemical coupling agent
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2319/00Fusion polypeptide

Abstract

The invention relates to a method for covalent immobilisation of a biomolecule to a solid material and products arising therefrom, comprising providing a solid material with an activated surface, preferably by treatment with an oxidising agent, and subsequently contacting said material with a mixture of biomolecule to be immobilised and one or more silane components, preferably present in a pre-mixed binding solution. The immobilisation procedure is particularly useful for immunoassays, enzyme-linked immunosorbent assays (ELISA), surface plasmon resonance immunoassays, microarrays or microfluidic assays.
PCT/EP2013/070919 2012-10-08 2013-10-08 One-step biomolecular immobilisation procedure and products thereof WO2014056896A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
EP13773780.5A EP2903740A2 (en) 2012-10-08 2013-10-08 One-step biomolecular immobilisation procedure and products thereof

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP12187638.7 2012-10-08
EP12187638 2012-10-08

Publications (2)

Publication Number Publication Date
WO2014056896A2 WO2014056896A2 (en) 2014-04-17
WO2014056896A3 true WO2014056896A3 (en) 2014-07-03

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2013/070919 WO2014056896A2 (en) 2012-10-08 2013-10-08 One-step biomolecular immobilisation procedure and products thereof

Country Status (2)

Country Link
EP (1) EP2903740A2 (en)
WO (1) WO2014056896A2 (en)

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CN105985440A (en) * 2015-02-06 2016-10-05 中国科学院苏州纳米技术与纳米仿生研究所 Three-dimensional graphene-cytokine composite system, preparation method and application thereof
DE102015003404B4 (en) * 2015-03-18 2021-10-07 Forschungszentrum Jülich GmbH Process for the production of a standard for the detection of protein aggregates of a protein misfolding disease and standard and its use
CN104897758A (en) * 2015-06-26 2015-09-09 王利兵 Method for manufacturing and modifying electrochemiluminescence electrode array
EP3317666B1 (en) * 2015-06-30 2020-10-07 IMEC vzw Surface immobilization of an analyte-recognizing molecule
CN104965076B (en) * 2015-07-14 2017-01-18 上海拜豪生物科技有限公司 Cadmium-chelated immune complex as well as preparation method and application thereof
EP3322410A4 (en) * 2015-07-14 2019-02-20 Nanosniff Technologies Pvt. Ltd. Process for immobilizing one or more receptor biomolecules on one or more solid surfaces
WO2017172879A1 (en) * 2016-03-29 2017-10-05 Oneighty°C Technologies Corporation Method for rapidly determining effective sterilization, demmunization, and/or disinfection
CN106335234B (en) * 2016-08-19 2018-10-19 中国科学院上海微系统与信息技术研究所 A kind of graphene albumen laminated film and preparation method based on non-covalent modification
CN106814116B (en) * 2017-02-20 2019-05-17 华南农业大学 A kind of unmarked type acrylamide electrochemical immunosensor and its construction method and application
CN109266641A (en) * 2018-09-27 2019-01-25 福建海峡石墨烯产业技术研究院有限公司 A kind of method and detecting electrode that enzyme is fixed on graphene based on glutaraldehyde
KR102550375B1 (en) * 2018-11-26 2023-07-04 주식회사 엘지화학 Biosensor and Preparation Method There of
CN113125708B (en) * 2019-12-31 2023-01-20 暨南大学 Microporous plate based on nuclear pore membrane and preparation method and application thereof
CA3181186A1 (en) * 2020-04-27 2021-11-04 CCOA Therapeutics Inc. Immobilized self-assembled protein multimers
CN112666299A (en) * 2020-12-29 2021-04-16 南通华叶生物科技集团有限公司 Cell membrane chromatographic column preparation method for efficiently screening specific medicines for bacterial infection
CN113419069B (en) * 2021-06-16 2022-07-15 东软威特曼生物科技(南京)有限公司 Anti-cyclic citrullinated peptide antibody detection kit and method
CN115015542B (en) * 2022-04-17 2023-02-28 吉林迅准生物技术有限公司 Respiratory tract bacterium combined detection device and preparation method thereof
CN115902208A (en) * 2022-09-07 2023-04-04 深圳前海石墨烯产业有限公司 Graphene film loaded polystyrene ELISA plate and preparation method thereof

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105498706A (en) * 2015-12-09 2016-04-20 广东研捷医药科技有限公司 Preparation method for protein A immunoaffinity column
CN105498706B (en) * 2015-12-09 2019-07-23 广东研捷医药科技有限公司 A kind of preparation method of albumin A immune affinity column

Also Published As

Publication number Publication date
EP2903740A2 (en) 2015-08-12
WO2014056896A2 (en) 2014-04-17

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