WO2014056896A3 - One-step biomolecular immobilisation procedure and products thereof - Google Patents
One-step biomolecular immobilisation procedure and products thereof Download PDFInfo
- Publication number
- WO2014056896A3 WO2014056896A3 PCT/EP2013/070919 EP2013070919W WO2014056896A3 WO 2014056896 A3 WO2014056896 A3 WO 2014056896A3 EP 2013070919 W EP2013070919 W EP 2013070919W WO 2014056896 A3 WO2014056896 A3 WO 2014056896A3
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- immobilisation
- products
- biomolecular
- biomolecule
- immunoassays
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54353—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals with ligand attached to the carrier via a chemical coupling agent
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
Abstract
The invention relates to a method for covalent immobilisation of a biomolecule to a solid material and products arising therefrom, comprising providing a solid material with an activated surface, preferably by treatment with an oxidising agent, and subsequently contacting said material with a mixture of biomolecule to be immobilised and one or more silane components, preferably present in a pre-mixed binding solution. The immobilisation procedure is particularly useful for immunoassays, enzyme-linked immunosorbent assays (ELISA), surface plasmon resonance immunoassays, microarrays or microfluidic assays.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
EP13773780.5A EP2903740A2 (en) | 2012-10-08 | 2013-10-08 | One-step biomolecular immobilisation procedure and products thereof |
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
EP12187638.7 | 2012-10-08 | ||
EP12187638 | 2012-10-08 |
Publications (2)
Publication Number | Publication Date |
---|---|
WO2014056896A2 WO2014056896A2 (en) | 2014-04-17 |
WO2014056896A3 true WO2014056896A3 (en) | 2014-07-03 |
Family
ID=47010351
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/EP2013/070919 WO2014056896A2 (en) | 2012-10-08 | 2013-10-08 | One-step biomolecular immobilisation procedure and products thereof |
Country Status (2)
Country | Link |
---|---|
EP (1) | EP2903740A2 (en) |
WO (1) | WO2014056896A2 (en) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN105498706A (en) * | 2015-12-09 | 2016-04-20 | 广东研捷医药科技有限公司 | Preparation method for protein A immunoaffinity column |
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CN105985440A (en) * | 2015-02-06 | 2016-10-05 | 中国科学院苏州纳米技术与纳米仿生研究所 | Three-dimensional graphene-cytokine composite system, preparation method and application thereof |
DE102015003404B4 (en) * | 2015-03-18 | 2021-10-07 | Forschungszentrum Jülich GmbH | Process for the production of a standard for the detection of protein aggregates of a protein misfolding disease and standard and its use |
CN104897758A (en) * | 2015-06-26 | 2015-09-09 | 王利兵 | Method for manufacturing and modifying electrochemiluminescence electrode array |
EP3317666B1 (en) * | 2015-06-30 | 2020-10-07 | IMEC vzw | Surface immobilization of an analyte-recognizing molecule |
CN104965076B (en) * | 2015-07-14 | 2017-01-18 | 上海拜豪生物科技有限公司 | Cadmium-chelated immune complex as well as preparation method and application thereof |
EP3322410A4 (en) * | 2015-07-14 | 2019-02-20 | Nanosniff Technologies Pvt. Ltd. | Process for immobilizing one or more receptor biomolecules on one or more solid surfaces |
WO2017172879A1 (en) * | 2016-03-29 | 2017-10-05 | Oneighty°C Technologies Corporation | Method for rapidly determining effective sterilization, demmunization, and/or disinfection |
CN106335234B (en) * | 2016-08-19 | 2018-10-19 | 中国科学院上海微系统与信息技术研究所 | A kind of graphene albumen laminated film and preparation method based on non-covalent modification |
CN106814116B (en) * | 2017-02-20 | 2019-05-17 | 华南农业大学 | A kind of unmarked type acrylamide electrochemical immunosensor and its construction method and application |
CN109266641A (en) * | 2018-09-27 | 2019-01-25 | 福建海峡石墨烯产业技术研究院有限公司 | A kind of method and detecting electrode that enzyme is fixed on graphene based on glutaraldehyde |
KR102550375B1 (en) * | 2018-11-26 | 2023-07-04 | 주식회사 엘지화학 | Biosensor and Preparation Method There of |
CN113125708B (en) * | 2019-12-31 | 2023-01-20 | 暨南大学 | Microporous plate based on nuclear pore membrane and preparation method and application thereof |
CA3181186A1 (en) * | 2020-04-27 | 2021-11-04 | CCOA Therapeutics Inc. | Immobilized self-assembled protein multimers |
CN112666299A (en) * | 2020-12-29 | 2021-04-16 | 南通华叶生物科技集团有限公司 | Cell membrane chromatographic column preparation method for efficiently screening specific medicines for bacterial infection |
CN113419069B (en) * | 2021-06-16 | 2022-07-15 | 东软威特曼生物科技(南京)有限公司 | Anti-cyclic citrullinated peptide antibody detection kit and method |
CN115015542B (en) * | 2022-04-17 | 2023-02-28 | 吉林迅准生物技术有限公司 | Respiratory tract bacterium combined detection device and preparation method thereof |
CN115902208A (en) * | 2022-09-07 | 2023-04-04 | 深圳前海石墨烯产业有限公司 | Graphene film loaded polystyrene ELISA plate and preparation method thereof |
Citations (3)
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EP1365249A1 (en) * | 2001-02-08 | 2003-11-26 | Ngk Insulators, Ltd. | Biochip and method of manufacturing the biochip |
US20050079486A1 (en) * | 2003-09-23 | 2005-04-14 | Wiscnsin Alumni Research Foundation - | Using liquid crystals to detect affinity microcontact printed biomolecules |
WO2010044083A2 (en) * | 2008-10-14 | 2010-04-22 | Dublin City University | A multi-well plate for biological assays |
Family Cites Families (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
IE20080934A1 (en) | 2007-11-22 | 2009-09-02 | Univ Dublin City | A method of immobilising biological molecules to a support and products thereof |
-
2013
- 2013-10-08 WO PCT/EP2013/070919 patent/WO2014056896A2/en active Application Filing
- 2013-10-08 EP EP13773780.5A patent/EP2903740A2/en not_active Withdrawn
Patent Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP1365249A1 (en) * | 2001-02-08 | 2003-11-26 | Ngk Insulators, Ltd. | Biochip and method of manufacturing the biochip |
US20050079486A1 (en) * | 2003-09-23 | 2005-04-14 | Wiscnsin Alumni Research Foundation - | Using liquid crystals to detect affinity microcontact printed biomolecules |
WO2010044083A2 (en) * | 2008-10-14 | 2010-04-22 | Dublin City University | A multi-well plate for biological assays |
Non-Patent Citations (9)
Title |
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CHANDRA KUMAR DIXIT ET AL: "Development of a High Sensitivity Rapid Sandwich ELISA Procedure and Its Comparison with the Conventional Approach", ANALYTICAL CHEMISTRY, vol. 82, no. 16, 15 August 2010 (2010-08-15), pages 7049 - 7052, XP055048836, ISSN: 0003-2700, DOI: 10.1021/ac101339q * |
DAN ZHENG ET AL: "Mediatorless amperometric glucose biosensing using 3-aminopropyltriethoxysilane-functionalized graphene", TALANTA, vol. 99, 15 May 2012 (2012-05-15), pages 22 - 28, XP055093586, ISSN: 0039-9140, DOI: 10.1016/j.talanta.2012.05.014 * |
DAN ZHENG ET AL: "Rapid and simple preparation of a reagentless glucose electrochemical biosensor", THE ANALYST, vol. 137, no. 16, 1 January 2012 (2012-01-01), pages 3800, XP055048865, ISSN: 0003-2654, DOI: 10.1039/c2an35128e * |
DIXIT CHANDRA KUMAR ET AL: "Multisubstrate-compatible ELISA procedures for rapid and high-sensitivity immunoassays", NATURE PROTOCOLS, NATURE PUBLISHING GROUP, GB, vol. 6, no. 4, 1 April 2011 (2011-04-01), pages 439 - 445, XP009166031, ISSN: 1750-2799 * |
S K VASHIST ET AL: "Rapid Immunodiagnostic Kits based on proprietary 1-step chemistry for covalent and leach-proof antibody immobilisation", MIKROSYSTEMTECHNIK - KONGRESS 2013, AACHEN, GERMANY, 14-16 OCT. 2013; POSTER 1.13, 15 October 2013 (2013-10-15), http://www.imtek.de/data/lehrstuehle/app/dokumente/conferences-pdf/conferences-2013/vashist-rapid-immunodiagnostic-kits, pages 384 - 387, XP055093601 * |
SANDEEP KUMAR VASHIST ET AL: "Effect of antibody immobilization strategies on the analytical performance of a surface plasmon resonance-based immunoassay", THE ANALYST, vol. 136, no. 21, 1 January 2011 (2011-01-01), pages 4431, XP055048842, ISSN: 0003-2654, DOI: 10.1039/c1an15325k * |
SANDEEP KUMAR VASHIST: "A sub-picogram sensitive rapid chemiluminescent immunoassay for the detection of human fetuin A", BIOSENSORS AND BIOELECTRONICS, vol. 40, no. 1, 1 February 2013 (2013-02-01), pages 297 - 302, XP055093588, ISSN: 0956-5663, DOI: 10.1016/j.bios.2012.07.067 * |
SANDEEP KUMAR VASHIST: "Comparison of 1-Ethyl-3-(3-Dimethylaminopropyl) Carbodiimide Based Strategies to Crosslink Antibodies on Amine-Functionalized Platforms for Immunodiagnostic Applications", DIAGNOSTICS, vol. 2, no. 3, 27 August 2012 (2012-08-27), pages 23 - 33, XP055093581, DOI: 10.3390/diagnostics2030023 * |
YANG LUO ET AL: "Sensitive and rapid quantification of C-reactive protein using quantum dot-labeled microplate immunoassay", JOURNAL OF TRANSLATIONAL MEDICINE, BIOMED CENTRAL, LONDON, GB, vol. 10, no. 1, 6 February 2012 (2012-02-06), pages 24, XP021118747, ISSN: 1479-5876, DOI: 10.1186/1479-5876-10-24 * |
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN105498706A (en) * | 2015-12-09 | 2016-04-20 | 广东研捷医药科技有限公司 | Preparation method for protein A immunoaffinity column |
CN105498706B (en) * | 2015-12-09 | 2019-07-23 | 广东研捷医药科技有限公司 | A kind of preparation method of albumin A immune affinity column |
Also Published As
Publication number | Publication date |
---|---|
EP2903740A2 (en) | 2015-08-12 |
WO2014056896A2 (en) | 2014-04-17 |
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