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Protocols. DEPC Water Treatment (OpenWetWare) Diethyl pyrocarbonate (DEPC) is an efficient, nonspecific inhibitor of RNases. It is typically used to treat water ...
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Feb 2, 2009 - Abstract: Solutions (water and other solutions) should be treated with 0.1% DEPC. DEPC is a strong, but not absolute, inhibitor of RNases.
Dec 16, 2011 - 1 post
I have seen that a lot of people use DEPC-treated PFA for fixing tissue that will be used for in situ hybridisation or will later want to extract RNA ...Jan 20, 2011 - 1 post
Hi all. If I prepared 0.1% DEPC treated water, then for how long i can keep it ,and when it'll be expired? and can i keep it at room temp ,and ...Jun 19, 2006 - Does anyone know DEPC can influence PH of water? I check the PH of redistilled water either before and after DEPC treated, the PH before ...
Mar 26, 2005 - For RNA extraction, we have to make DEPC-water and DEPC-PBS. I want to ask how do you guys usually prepare these solutions? 1. clean ...
Feb 17, 2009 - 1 post
I have strange QPCR results lately and I'm assumping that there's contamination in my DEPC-water which is also used to prepare the ...Nov 9, 2012 - 2 posts
By the time you use DEPC treated reagents, the chemical has already degraded into CO2, ethanol, and water. If it's not present, then RNases ...Oct 25, 2006 - can you please tell me if it is possible to treat EDTA with DEPC? Is there a list anywhere were you can look up which substances can be treatet ...
Mar 20, 2006 - Speaking of RNA extraction/handling is there anybody who does not use this DEPC treatment? Is there any substitute for DEPC to preserve ...