Tetracycline-dependent translational regulation could be imposed via specific aptamers inserted at two different positions in the 5' untranslated region (5'UTR) ...
Aug 11, 2003 · The addition of tetracycline facilitates the formation of a tetracycline-aptamer complex which interferes with different steps of translation ...
We have identified a tetracycline (tc)-binding aptamer which confers tc-dependent gene regulation in yeast (14).
Jul 10, 2009 · The tc aptamer has been used to control gene expression on different levels, including translation initiation (16,26) and pre-mRNA splicing (27) ...
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Jun 23, 2023 · (A) In yeast, positioning of the riboswitch construct within the 5' UTR of the mRNA determines the type of translation regulation in the ...
(B) Regulation of mRNA splicing is achieved by using the tetracycline aptamer to control accessibility of the 5' splice site (SS). Incorporation of the 5' SS ...
We have shown that aptamers isolated by SELEX for high affinity binding to tc can be used as a molecular switch to regulate gene expression at the level of ...
By introducing the aptamer into two introns of a yeast mRNA, they achieved 32-fold tetracycline-mediated repression of gene expression in vivo (Weigand and ...
Nov 21, 2023 · In this work, we used an RNA aptamer activated by tetracycline to modulate protein production at the translational level.
Efficient regulation is obtained when the aptamer is inserted close to the 5′splice site (SS) with the consensus sequence of the SS located within the aptamer ...